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Analysis of bilirubin's relevant photoproducts and their biological significance in the issue of neonatal jaundice
Křepelka, David ; Kozlík, Petr (advisor) ; Zelenka, Jaroslav (referee)
Neonatal jaundice occurs in almost 60% of full-term and 80% of premature babies, where a slightly increased concentration of bilirubin protects against oxidative stress just after birth. When a specific bilirubin level is exceeded in serum (usually above 340 µmol·l-1 ), bilirubin-could induce kernicterus. These negative states are prevented by blue-green light phototherapy (420-510 nm), which converts bilirubin into more polar photoproducts that are more easily excreted via bile and/or urine. Published data have shown that newborns with indicated phototherapy may develop clinical problems later in life (higher incidence of e.g. asthma, allergies, type 1 diabetes was observed at a later age). A possible reason for the occurrence of these diseases is the specific biological activity of photoproducts. This study aims to purify an unknown photoproduct (band P), formed after 8 hours of irradiation of a bilirubin solution with blue light, and to establish a quantitative analytical method for its measurement in relevant matrices. This product was isolated and separated by a thin layer and subsequent column flash chromatography. In the next part, an LC-MS/MS method was developed for quantification of band P. Finally, urine, plasma, and feces samples collected from 15 newborns before and after phototherapy...
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The effect of age on selected prenatal outcomes and breastfeeding
Fontániová, Simona ; Lukšová, Markéta (advisor) ; Tomášková, Zuzana (referee)
: For my bachelor's thesis, I chose the topic Influence of age on selected prenatal outcomes and breastfeeding. The thesis contains a theoretical part and a practical part. In the theoretical part, I try to explain the difficulties associated with pregnancy. In the first chapter, I focused on high-risk pregnancy and childbirth. Subsequently, disorders of the duration of pregnancy, which include miscarriage, premature birth, ectopic pregnancy and post-term pregnancy. In the second chapter, I continue with the importance of breastfeeding and, at the same time, breastfeeding problems that can trouble the mother. In the third chapter, I deal with the classification of newborns and birth injuries. For my practical part of the research work, I chose a questionnaire that reached mothers of different age categories via the Internet. I believe that my bachelor's thesis will benefit all women who are not aware of difficulties in pregnancy at an older age. Based on research, I found that age plays an important role in a woman's life during pregnancy. A woman's age has a negative impact both on the ability to get pregnant and on the birth itself and subsequent breastfeeding. Keywords : Breastfeeding, high-risk pregnancy, advanced maternal age, pregnancy, labor, maternal outcomes, fetal outcomes, birth weight,...
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Study of bilirubin and its oxidation products
Capková, Nikola ; Dvořák, Aleš (advisor) ; Vejražka, Martin (referee) ; Holendová, Blanka (referee)
Phototherapy (PT) with blue-green light (420-490 nm) is the standard treatment for severe neonatal jaundice to prevent infants from toxic bilirubin (BR). Upon blue-green light exposure, BR is converted to more polar photoisomer (PI) lumirubin (LR) and the other oxidation products (mono-, di-, tripyrrols) which can be more easily disposed of the body via urine and/or bile. Although generally considered to be safe, PT is accompanied by an increased risk of various pathophysiological conditions (inflammatory processes, allergies, diabetes, and some types of cancer), in extremely low-birth-weight newborns. Thus, to account for these consequences, our study aimed to understand the mechanism of BR secretion in different tissues and cell lines and investigate the bioactive properties of BR and its main photooxidation product LR. At first, we focused on the detection of BR in the bile and feces of hyperbilirubinemic Gunn rats. Simultaneously, we tested the antioxidant and pro-oxidant effects of unconjugated BR in human hepatoblastoma (HepG2), proximal tubular (HK2), neuroblastoma (SH-SY5Y), and murine endothelial (H5V) cells by exposing them to progressively increasing concentrations of BR. To compare the BR and LR effects on metabolic and oxidative stress markers, the biological activities were...
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The biological role of the metabolic products of haem and bilirubin.
Jašprová, Jana ; Vítek, Libor (advisor) ; Farghali, Hassan (referee) ; Mičuda, Stanislav (referee)
Present work has been focused on the importance of the products of the heme catabolic pathway, in particular under conditions of unconjugated hyperbilirubinemias (neonatal jaundice and Crigler-Najjar syndrome (CNS)). The second part of the project was focused on the improvement of some pharmacological approaches used in the treatment of these diseases, as well as on studies of bilirubin products that are formed during the treatment by phototherapy (PT). Neonatal jaundice is one of the most common complications in neonates. Currently, there is no efficient pharmacotherapy and the treatment with blue light is used as a gold standard for severe neonatal jaundice. However, the absolute safety of PT has still not been confirmed. In this context, it is important to note that some neonatologists start the PT before serum bilirubin levels reach the recommended values and that patients with CNS type I (CNSI) are forced to be on life-long PT (unless undergoing liver transplantation). The focus of the present project was to study biological effects of bilirubin photoisomers (PI) in an in vitro model of the human neuroblastoma SH-SY5Y cells that are used for studies of the neuronal metabolism. In further studies performed on animal model of hyperbilirubinemic rats and mice, we investigated a suitable gene...
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The nursing care of newborns with Hyperbilirubinaemia
KUCHAŘOVÁ, Petra
This thesis is focused on the nursing care of newborns with neonatal jaundice. Given the focus of the work, the theoretical part describes the newborn, bilirubin, neonatal jaundice, its diagnosis, treatment, nursing care during hospitalization and neonatal care after discharge to home care. The research was conducted with nurses or midwives in the neonatal wards during the months of March and April 2018. A qualitative method was used to conduct the research section. The data collection was conducted through semi-structured interviews. The interviews were conducted after prior arrangement and with the approval of the head nurses. The interview contained 12 open questions. Data analysis was performed by hand coding, also called the pencil and paper method. Responses by individual respondents were identified by codes that were then categorized under different subcategories. As a supplementary method of research, observation was used. It contained 8 open questions and an observation sheet, all of which were done after prior agreement and consent of the mothers of newborns. One goal was set for this thesis: to map the nursing care of newborns with neonatal jaundice. Based on this goal, a research question focused on how midwives and pediatric nurses care for newborns with neonatal jaundice. The research showed that all respondents have an overview of the issue, and that the information they have is subsequently carried out into practice. The results of this research can serve as informative material for midwives and nurses who want to improve their care of newborns with neonatal jaundice, or who are currently preparing to work in this profession.
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The biological role of the metabolic products of haem and bilirubin.
Jašprová, Jana ; Vítek, Libor (advisor) ; Farghali, Hassan (referee) ; Mičuda, Stanislav (referee)
Present work has been focused on the importance of the products of the heme catabolic pathway, in particular under conditions of unconjugated hyperbilirubinemias (neonatal jaundice and Crigler-Najjar syndrome (CNS)). The second part of the project was focused on the improvement of some pharmacological approaches used in the treatment of these diseases, as well as on studies of bilirubin products that are formed during the treatment by phototherapy (PT). Neonatal jaundice is one of the most common complications in neonates. Currently, there is no efficient pharmacotherapy and the treatment with blue light is used as a gold standard for severe neonatal jaundice. However, the absolute safety of PT has still not been confirmed. In this context, it is important to note that some neonatologists start the PT before serum bilirubin levels reach the recommended values and that patients with CNS type I (CNSI) are forced to be on life-long PT (unless undergoing liver transplantation). The focus of the present project was to study biological effects of bilirubin photoisomers (PI) in an in vitro model of the human neuroblastoma SH-SY5Y cells that are used for studies of the neuronal metabolism. In further studies performed on animal model of hyperbilirubinemic rats and mice, we investigated a suitable gene...
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